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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of this gene continues to be observed in several tumors, which ends up in chromosome instability, and thus may add to tumorigenesis. This protein was located to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a important part in marketing mobile-cycle progression within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, have been explained. Two extra splice variants were documented but detailed nucleotide sequence info is not still obtainable.

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Blocking peptides are peptides that bind particularly to your target antibody and block antibody binding. These peptide commonly consists of the epitope identified via the antibody.

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This manual outlines further more feasible optimization of mobile and immunoassay parameters to make certain the very best benefits are...

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This guidebook outlines even more doable optimization of mobile and immunoassay parameters to be certain the very best effects are...

Antibodies sure to the blocking peptide no longer bind on the epitope over the target protein. By evaluating the staining from your blocked antibody compared to the antibody on your own, one can see which staining is specific.

Concentrate on Information and facts Cyclin E belongs into the remarkably conserved cyclin relatives, whose customers are characterized by a dramatic periodicity in protein abundance through the mobile cycle. Cyclins purpose as regulators of CDK kinases. Different cyclins exhibit distinctive expression and degradation styles which lead towards the temporal coordination of each and every mitotic celebration. Cyclin E types a fancy with and capabilities to get more info be a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates at the G1-S period boundary and it is degraded as cells development by way of S phase.

The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by equally antibodies...

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